Fig. 2

ANXA2- and OSMR-mediated regulation of cell proliferation, invasion, and mesenchymal transition in vitro. a, b qRT-PCR (a) and western blot analysis (b) of ANXA2 and OSMR expression in MGG23 cells with or without ANXA2 overexpression and concomitant OSMR knockdown. c qRT-PCR analysis of mesenchymal signature gene expression in MGG23 cells (n = 3). d WST-1 cell proliferation assay of MGG23 cells after 2 days incubation. Data were normalized to the control cells (n = 10). e, f| Matrigel invasion assay of MGG23 cells after 10 days. Data were normalized to the control cells (n = 4). Scale bar, 200 μm. Data are shown as the mean ± SEM. **P < 0.01, ***P < 0.001 by one-way ANOVA with Bonferroni’s post hoc test