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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Severe hydroxymethylbilane synthase deficiency causes depression-like behavior and mitochondrial dysfunction in a mouse model of homozygous dominant acute intermittent porphyria

Fig. 5

Analysis of adult hippocampal neurogenesis in HMBS-deficient mice. a BrdU injection schedules for the proliferation and survival paradigms. b Quantification of hippocampal progenitor cell proliferation (BrdU+ cell count) and c respective fluorescence microscope images in HMBS-deficient (KI) and wildtype (WT) mice (BrdU+ cells in green). d Survival of newborn (BrdU+) cells over a two-week period. e Relative differentiation rates of newborn (BrdU+ cells) cells into astrocytes (GFAP+ cells) and f mature neurons (NeuN+ cells). g Developmental stages of doublecortin stained DCX+ (green) BrdU+ (red) labeled cells according to defined morphological criteria (proliferative, intermediate and postmitotic). h Quantification (%) of BrdU+ cells in each developmental stage in KI and WT mice. All values are presented as mean ± SEM; n = 11–13 per genotype/3 sections per mouse. Statistical significances displayed are results of Student’s t-tests. ***p < 0.001, **p < 0.01, *p < 0.05; HMBS, hydroxymethylbilane synthase; BrdU, 5-bromo-2′-deoxyuridine; GFAP, glial fibrillary acidic protein; NeuN, neuronal nuclei; DCX, doublecortin

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