Fig. 3

Immunohistochemical evaluation of myelin proteins in the hippocampus of HMBS-deficient mice. a Evaluation of MOG intensity and b microscopal image of MOG staining in KI and WT mice, c PLP1 intensity and d microscopal image of PLP1 staining in KI and WT mice. e Number of CNP+ cells and f microscopal image of CNP staining in KI and WT mice. All values are presented as mean ± SEM; n = 7–12 per genotype/3 sections per mouse. Statistical significances displayed are results of Student’s t-test. *p < 0.05, ***p < 0.001; HMBS, hydroxymethylbilane synthase; MOG, myelin oligodendrocyte glycoprotein; PLP1, myelin proteolipid protein 1; CNP, 2′,3′-Cyclic-nucleotide 3′-phosphodiesterase