Fig. 7

Accumulation of oligomeric tau in 6-mo Tg mice does not lead to detectable level of apoptotic cell death. Representative images of sagittal CA1 sections from 6- and 12-mo Wt and Tg mice hippocampi (n = 3 for each category), labeled with antibodies against the phospho-tau (AT8), and activated (cleaved) caspase-3 antibodies. Immunofluorescent signals were analyzed by LSC laser scanning confocal microscopy (z projection). Nuclei were visualized with DAPI staining. The scale bars represent 20 μm. b Quantification of nuclei (DAPI staining) per surface unit within CA1 sections from 6- and 12-mo Wt and Tg mice hippocampi (n = 10 for each mouse category). DAPI signals were analyzed by LSC laser scanning confocal microscopy (z projection). Graph shows the mean of nuclear fluorescence per mouse. Data are presented as mean ± SEM (***P < 0.001). c WB analysis of CA1 lysates from 6- and 12-mo mice and hippocampi for pro-caspase-3 and activate cleaved caspase-3. Actin was used as loading control