Fig. 3

Intrathecal MIS416-induced neutrophils inhibited progression of EAE. Mice were immunized with MOG p35–55 to induce EAE and clinical signs were scored daily. a Flowchart showing the experimental design. b Clinical scores of mice with EAE. Shown is pooled data from three individual experiments. Mice treated by intrathecal administration of MIS416 (n = 17), at EAE onset, progressed less rapidly following 2 days than control animals(n = 12). c Cells isolated from brains of mice with EAE were analyzed by flow cytometry, at 24 h post intrathecal administration of MIS416 (n = 8–19). Bar graph shows MIS416-induced recruitment of CD45^high cells in the CNS. d, e Monocytes or neutrophils were sorted from the CNS of normal mice or mice with EAE 1 day after they received intrathecal MIS416. Cells were transferred to mice on the day of first onset of EAE. d Clinical score of disease progression in mice that received intrathecal injection of neutrophils and monocytes that were sorted from normal mice (n = 9–14 per group). e Clinical scores of mice that received intrathecal injection of neutrophils and monocytes sorted from donor mice with EAE (n = 3–7 per group), that had received intrathecal MIS416 at the onset of disease, 24 h previously. f Images show H&E - and LFB staining of spinal cord sections from mice with EAE, 1 day after receiving either intrathecal MIS416, Neutrophils and monocytes induced by MIS416. Red boxes show higher magnification of selected areas of spinal cord sections stained with H&E staining (arrows point at parenchymal infiltrates) and corresponding areas with LFB staining (arrows point at areas with loss of LFB). Scale bars 100 μm. Data are presented as mean ± SEM. Results were analyzed using the two-tailed Mann-Whitney u-test; * p < 0.05, ** p < 0.01, *** p < 0.001