Table 4 List of Oligonucleotide Sequences
ID | Sequence (5’-3’) | Purpose |
|---|---|---|
SOD1-G93A-gRNA-fwd | TAATACGACTCACTATAGGAATCTTCAATAGACACA | gRNA DNA template for gRNA synthesis |
SOD1-G93A-gRNA-rev | TTCTAGCTCTAAAACATGTGTCTATTGAAGATTC | |
SOD1-G93A-ssODN | p-ZOCATCTGATGCTTTTTCATTATTAGGCATGTTGGAGAC TTGGGCAATGTGACTGCTGACAAAGATGCTGTGGCAGAT GTGTCTATTGAAGATTCZET | knock-in donor DNA |
OT1-fwd | AGACACTGGCATTTCAGACGTAGG | Off-target analysis |
OT1-rev | AGAAGACCCATGATTCCTGGGC | |
OT2-fwd | CAAAGGTAGATCGCCAGAGACAAC | |
OT2-rev | TGCCTGGTGGGAAGGTCCAT | |
OT3-fwd | CAACCGGAGAAGTGACAAGGC | |
OT3-rev | CTCAGATCAGTCTCTAGCAATCCC | |
OT4-fwd | TCTGTAGTCAGCCTCTGTCCAC | |
OT4-rev | CTTTCTGGGTCCCCAGTTTCTAC | |
OT5-fwd | GGGCCTATGGAAGTGAACTCAAC | |
OT5-rev | ATGTTGCATGTTTCTATGATGGGCCTATCC | |
OT6-fwd | TCCTGGATGGTCCTGGACATCA | |
OT6-rev | AGCAATGTGTGTCACTCCAGGATC | |
OT7-fwd | GCAAATAAGCAACAGACCGAGGAG | |
OT7-rev | GCCTCAGCTTTCTCATGTACCATC |