Table 2 Human ALS & Control DNA Methylation Pyrosequencing Targets
Aprataxin | |
 Gene symbol | APTX |
 Entrez Gene ID | 54,840 |
 Sequenced strand | Sense |
 Amplicon size | 253 |
 Biotin modification | reverse PCR primer |
 Sequence to analyze | ACGCAAAGTGGGTCGAAGACCAACGCGAGCGCCCG |
 Sequence after bisulfite | AYGTAAAGTGGGTYGAAGATTAAYGYGAGYGTTYG |
 Number of CpG sites | 6 |
 Nucleotide dispensation order | TATCGTCAATGATGTCGAGAGTATCGTCGAGTCG |
8-oxoguanine DNA glycosylase | |
 Gene symbol | OGG1 |
 Entrez Gene ID | 4968 |
 Sequenced strand | Sense |
 Amplicon size | 253 |
 Biotin modification | reverse PCR primer |
 Sequence to analyze | CCGTGTGGGCGAGGCCTTAAGGGTCGTGGTCCTTGTCTGGGCGGGGT |
 Sequence after bisulfite | TYGTGTGGGYGAGGTTTTAAGGGTYGTGGTTTTTGTTTGGGYGGGGT |
 Number of CpG sites | 4 |
 Nucleotide dispensation order | ATCGTGTAGTCGATGTTAGTCGATGTTGTCTGTCG |
Apurinic/apyrimidinic endonuclease 1 | |
 Gene symbol | APEX1 |
 Entrez Gene ID | 328 |
 Sequenced strand | Sense |
 Amplicon size | 92 |
 Biotin modification | reverse PCR primer |
 Sequence to analyze | GTCCGCGCTGGGCCGCAGCTTTCCGGAGCGT |
 Sequence after bisulfite | GTTYGYGTTGGGTYGTAGTTTTTYGGAGYGT |
 Number of CpG sites | 5 |
 Nucleotide dispensation order | TGTCGTCGTCTAGTCGATAGTTCGAGTCG |
Polynucleotide kinase 3’-phosphatase | |
 Gene symbol | PNKP |
 Entrez Gene ID | 11,284 |
 Sequenced strand | Antisense |
 Amplicon size | 98 |
 Biotin modification | reverse PCR primer |
 Sequence to analyze | CGGAGGATCCAGTCCCCGCTACCGGCCTGAGCCTCGCGT |
 Sequence after bisulfite | YGGAGGATTTAGTTTTYGTTATYGGTTTGAGTTTYGYGT |
 Number of CpG sites | 5 |
 Nucleotide dispensation order | GTCGAGAGTAGATTCGTCTGATCGTGAGTTCGTCG |