Fig. 6

Treatment with T₃ 50 μg/kg is associated with downregulation of GABA synthesis in the infarct core and peri-infarct area 14 days after photothrombosis (PT) and the activity of cortical parvalbumin immunoreactive cells (PV⁺) in the ipsilateral and contralateral areas. a and b Immunohistochemistry NiDAB (c-fos) counterstained with NovaRed (PV) was performed to count PV⁺ c-fos⁺ (white arrows) and PV⁺ (black arrows) immunoreactive cells, in the ipsilateral and contralateral motor and somatosensory areas of mice treated with Vehicle (Vh) or T₃ 50 μg/kg. Scale bars 100 μm (a and b) and 20 μm (a’ and b’). c Levels of glutamate decarboxylase (GAD) 65/67 was downregulated in mice treated with T₃ 50 μg/kg, in the peri-infarct area 14 days after PT. Vh (n = 6 for PT and n = 3 for sham), T₃ 50 μg/kg (n = 6 for PT and n = 3 for sham). For uncropped images of western blots see Additional file 1: Figure S9. Results are displayed as means ± SEM. Statistical analysis was performed with two-tailed unpaired Student’s t test. d Functional recovery after T₃ 50 μg/kg treatment may be related to a decrease in the ratio between PV⁺ c-fos⁺ / PV⁺ immunoreactive cells observed in the ipsilateral area and the correspondent region in the contralateral hemisphere. Vh (n = 7), T₃ 50 μg/kg (n = 6), T₄ 50 μg/kg (n = 3). Results are displayed as means ± SEM. Statistical analysis was performed by One-way ANOVA and Bonferroni’s multiple comparisons test. Two-tailed unpaired Student’s t test was employed to determine p values