Fig. 3

Secernin-1 significantly accumulates in neurofibrillary tangles and dystrophic neurites in AD. a Hippocampus sections were immunostained with α-SCRN1 (green) and PHF1 antibody (red) in all three groups. b PHF1% load in High, Moderate and Low/no NFTs groups. c-d Quantification of staining in the hippocampus and adjacent temporal cortex showed similar basal levels of SCRN1 across the three groups that were not significantly altered by the presence of NFT pathology. In contrast, the amount of SCRN1 in NFTs was significantly higher than that present outside of NFTs in all three groups in hippocampus (c) and temporal cortex (d). **** p-value < 0.0001; ** p-value < 0.01; one-way ANOVA, Tukey’s multiple comparisons test. Scale bar: 25 μm