Fig. 3

N368-cleaved Tau is not detected in Lgmn knockout mouse brain total homogenate. a. Scheme of the workflow for the quantification of Tau from mouse total brain extracts using LC-MS/MS. b-c. Mass spectrometry quantification of 354–368 (b) and 354–369 (c) Tau peptides as a mesure of N369-cleaved and uncleaved Tau, respectively, in forebrain tissue from Lgmn^−/− (n = 9), Lgmn ^+/− (n = 10) and Lgmn^+/+ (n = 11) littermates at 2 months of age. b. Levels of N368-cleaved Tau are significantly lower in Lgmn ^+/− compared to Lgmn^+/+ mice (p = 0.0070) and are below LLOQ in Lgmn^−/− (p < 0.0001 vs Lgmn^+/− and Lgmn^+/+). c. Levels of uncleaved Tau were similar across genotypes (p = 0.153 Lgmn^+/− vs Lgmn^−/−; p = 0.494 Lgmn^+/+ vs Lgmn^−/−; p = 0.597 Lgmn^+/− vs Lgmn^+/+). In all graphs, data points, mean and standard deviation are shown. **p < 0.01