Fig. 6
In the mPFC ABET caused a long-lasting reduction of PV+ neurons and transient myelin reduction. Representative images for PV (green in merge), MBP (red in merge) and Hoechst (blue in merge) co-staining in the mPFC (Bregma 1.98) of the control (a) and EtOH (b) adolescent mice. Signals are inverted in gray scale images. c Summary of PV+ cell body density in the mPFC. d Summary of MBP+ myelin density in the mPFC. e Summary of PV and MBP correlation in the mPFC. Two-way ANOVA followed by post hoc Tukey’s test: ***, p < 0.001; **, p < 0.01; *, p < 0.05. f Confocal images for PV and MBP staining in the mPFC of control (upper) and ABET (EtOH, lower) adolescent mouse. Arrowheads, PV+ axons colocalizing with MBP staining. Scale bars, 300 μm in (a) and (b), 50 μm in (f)