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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Dynein activating adaptor BICD2 controls radial migration of upper-layer cortical neurons in vivo

Fig. 6

Depletion of BICD2 in RGPs in vivo does not decrease RGP division but alters the position and cell-cycle progression of dividing progenitor cells. a. Coronal cryo-sections of E14.5 cortices from Bicd2fl/fl;Emx1-Cre+/− (=Emx1-KO) and Bicd2fl/fl;Emx1-Cre−/− (=Emx1-WT) mice were stained against the apical progenitor marker PAX6 (red) and the RGP proliferation marker Phospho-Histone H3 (PH3) marker (green). DAPI is shown in blue. Scale bars in left panels are 100 μm; and 50 μm in the zooms. b. Graphical representation of the relative position of PH3+ cells over the cortical longitude from ventricular (VS) to pial surface (PS) (in %). c. Number (10− 4) of PH3+ cells per μm2 (N = 9–12, n = 20–72). d. Number (10− 4) of PH3+ cells per μm2 at the VS and sub-apical locations (N = 9–12, n = 20–72). e. Graphical representation of the relative position of PAX6 + cells over the cortical longitude from VS to PS (in %). f. Coronal cryo-sections of E14.5 cortices from Emx1-KO and Emx1-WT mice were stained against the basal intermediate progenitor marker TBR2 (red) and PH3 (green). DAPI is shown in blue. Scale bars in left panels are 100 μm; and 50 μm in the zooms. g. Graphical representation of the relative position of TBR2+ cells over the cortical longitude from VS to PS (in %). h. Relative amount of PH3+ cells positive or negative for PAX6 which divide at the VS or at sub-apical positions in the upper VZ/SVZ (N = 4–7, n = 20–72). i. Relative amount of PH3+ cells positive or negative for TBR2 which divide at the VS or at sub-apical positions in the upper VZ/SVZ (N = 4–6, n = 40–67). j. Number of PAX6+ cells as a ratio of the number of DAPI+ cells (N = 4–7, n = 20–72). k. Number of TBR2+ cells as a ratio of the number of DAPI+ cells (N = 4–6, n = 40–67). CP: cortical plate, PS: pial surface, SVZ: subventricular zone, VS: ventricular surface, VZ: ventricular zone. s.-ap: sub-apical. *** p < 0.001, ** p < 0.005, ns = not significant; error bars are ±SEM. Used tests: Unpaired t-test (c, k), One Way ANOVA with Tukey’s multiple comparisons (d), One Way ANOVA with Sidak’s multiple comparisons (h, i), Mann Whitney U test (j)

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