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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Dynein activating adaptor BICD2 controls radial migration of upper-layer cortical neurons in vivo

Fig. 4

The SMALED2B-associated Bicd2 point mutation fails to rescue neuronal migration defects. Ex vivo brain electroporation with MARCKS-GFP and BICD2 point mutants (GFP-BICD2_S107L (S107 L), GFP-BICD2_R694C (R694C), GFP-BICD2_K758M (K758M), GFP-BICD2_E774G (E774G)), at E14.5, followed by organotypical slice cultures for 4 DIV of cell-type-specific conditional Bicd2 KO mice and their control littermates - Bicd2fl/fl;Nex-Cre+/− (=Nex-KO) and Bicd2fl/fl;Nex-Cre−/− (=Nex-WT). a. Selected zooms of organotypical coronal slices, and corresponding relative position of GFP+ soma (squares) and endfeet (triangles) over the cortical longitude from ventricular to pial surface, of Nex-WT and Nex-KO at E14.5 + 4 DIV transfected with MARCKS-GFP and indicated BICD2 point mutants. Scale bars are 50 μm. b. Relative position of GFP+ soma (squares) and endfeet (triangles) over the cortical longitude from ventricular to pial surface for Nex-WT and Nex-KO transfected with indicated BICD2 point mutants (N = 3–10, n = 6–197). c. Average length of the leading edges in % of total cortical radial diameter from ventricular to pial surface for Nex-WT and Nex-KO transfected with indicated BICD2 point mutants (N = 3–10, n = 6–197).* p < 0.05, ns = not significant; error bars are ±SEM. Used tests: One Way ANOVA with Dunnet’s multiple comparisons (b (cell bodies), c), Kruskal Wallis test with Dunn’s multiple comparisons (b (endfeet))

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