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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Dynein activating adaptor BICD2 controls radial migration of upper-layer cortical neurons in vivo

Fig. 3

BICD2 is required for nuclear migration in upper-layer cortical neurons in locomotion mode. Ex vivo brain electroporation with MARCKS-GFP at E14.5, followed by organotypical slice cultures for 4 DIV of cell-type-specific conditional Bicd2 KO mice and their control littermates - Bicd2fl/fl;Nex-Cre+/− (=Nex-KO), Bicd2fl/fl;Emx1-Cre+/− (=Emx1-KO), Bicd2fl/fl;Nex-Cre−/− (=Nex-WT) and Bicd2fl/fl;Emx1-Cre−/− (=Emx1-WT) - respectively. a. Organotypical coronal slices of Nex-WT, Nex-KO, Emx1-WT and Emx1-KO mice at E14.5 + 4 DIV. Cells are labeled with MARCKS-GFP through ex vivo electroporation. Scale bars are 100 μm. b. Selected areas from images shown in (a). Scale bars are 50 μm. c. Graphical representation of the relative positions of GFP+ soma (circles), leading edges (lines) and endfeet (triangles) over the cortical longitude from ventricular (VS) to pial surface (PS) (in %) and 156.3 μm in width (in %). d. Organotypical coronal slices of Nex-WT, Nex-KO, Emx1-WT and Emx1-KO mice at E14.5+ 4 DIV. Cells are transfected with full-length GFP-BICD2 (BICD2_FL) and MARCKS-GFP through ex vivo electroporation. Scale bars are 100 μm. e. Selected areas from images shown in (d). Scale bars are 50 μm. f. Graphical representation of the relative positions of GFP+ soma (circles), leading edges (lines) and endfeet (triangles) over the cortical longitude from ventricular (VS) to pial surface (PS) (in %) and 156.3 μm in width (in %). g + i. Relative position of GFP+ soma (squares) and endfeet (triangles) over the cortical longitude from VS to PS for Nex-WT and Nex-KO (g) (N = 5–8, n = 21–148), and Emx1-WT and Emx1-KO mice (i) (N = 4–11, n = 29–151). h + j. Average length of the leading edges in % of total cortical radial diameter from VS to PS for Nex-WT and Nex-KO (h), and Emx1-WT and Emx1-KO mice (j). PS: pial surface, VS: ventricular surface. ** p < 0.005, * p < 0.05, ns = not significant; error bars are ±SEM. Used tests: One Way ANOVA with Dunnet’s multiple comparisons (g, h, i, j)

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