Fig. 5

Drp1 inhibition improves lysosomal function and inhibits mTOR activity. a - c Hela autophagy reporter cells were transfected with siRNA-Drp1 or scramble control and then treated with 25 μM chloroquine (CQ) overnight (16 h) a Cells were immunostained with an Drp1 antibody. Images were captured and autophagosome/ autolysosome quantification b & c was performed using ImageJ as described above. Data represent mean ± SEM (n = 3 independent experiments). One-Way ANOVA with Newman–Keuls post-hoc analysis * p < 0.05; compared to the vehicle control group. d & e Stable N27 cells were transfected with siRNA-Drp1, and then induced with PonA (20 μM) next day to induce α-syn expression. 48 h after, cells were collected and lysed for western blot analysis d. Phospho-4E-BP1 was probed and normalized to β-actin e Data represent mean ± SEM (n = 4–5 independent experiments), One-way ANOVA followed by Newman–Keuls post-hoc testing * p < 0.05; compared to the control group