Fig. 2

Chronic neuronal activity modulation in the SN aggravates α-SYN induced behavioral deficits without affecting dopaminergic neuronal loss. a Schematic representation of experimental set-up. b Detection of unilateral motor deficits using the cylinder test at different time points p.i (n = 5 to 13, Mean ± SD, two-way ANOVA Bonferroni Multiple Comparison test, *p < 0.05, **p < 0.01, ***p < 0.0001). c Representative images of TH staining in the midbrain (scale bar 1 mm) and stereological quantifications of DN in the injected and non-injected SNpc (n = 5 to 6, Mean ± SD, one-way ANOVA Bonferroni Multiple Comparison test, **p < 0.01). d Representative images of TH staining in the striatum (scale bar 2 mm). Striatal volume loss was calculated using FIJI software, in relation to the non-injected side (n = 5 to 6, Mean ± SD, one-way ANOVA Bonferroni Multiple Comparison test, *p < 0.05)