Table 1 Immunohistochemistry of histiocytic neoplasms
Antibody (Source) | Clone | Dilution | Antigen Retrieval (Ventana proprietary reagents) | Detection (Ventana proprietary reagents) |
|---|---|---|---|---|
CD163 (Novocastra) | 10D6 | 1:200 | uCC1 mild | iView DAB |
CD68 (Dako) | PG-M1 | 1:100 | uCC1 mild | iView DAB |
CD14 (Cell Marque) | EPR3653 | 1:100 | uCC1 standard | Optiview DAB |
Factor XIIIα (GeneTex) | Polyclonal | 1:250 | Protease | iView DAB |
Fascin (Dako) | 55 K-2 | 1:500 | uCC1 mild | iView DAB |
Ki-67 (Dako) | MIB-1 | 1:25 | uCC1 mild | iView DAB |
S100 (Dako) | Polyclonal | 1:3000 | uCC1 mild | iView DAB |
CD1α (Immunotech) | O1O | 1:5 | uCC1 mild | uV DAB |
Langerin (Leica) | 12D6 | 1:100 | uCC1 standard | uV DAB |
BRAF V600E (Ventana Medical Systems) | VE1 | Pre-dilute | uCC1 standard | OptiView DAB |