Fig. 2

CTRP3 levels are reduced in tibialis anterior muscle and blood plasma of SMA mice. Representative Western blots and quantification of CTRP3 levels in SMA mice. a tibialis anterior (WT N = 6; SMA N = 5), b gastrocnemius (N = 5) and c masseter (N = 4) muscles of P7 WT and SMA mice. ACTB was used as a loading control. d Immunofluorescence staining of an cross section of gastrocnemius muscle of a P7 WT mouse, using anti-Laminin α2 (red), anti-CTRP3 (green) and DAPI (blue). Scale bars = 200 μm. e Representative Western blots and quantification of blood plasma proteins isolated from P7 WT and SMA mice. CTRP3 levels were normalized to the total protein amount (stainfree gel) (N = 9). Dot graphs represent data from independent experiments and the bar graphs depict the mean ± s.d. Two-tailed unpaired student’s t-test was used to determine statistical signaficance. ** p < 0.01, n.s = not significant, p > 0.05