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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Sarm1 deletion suppresses TDP-43-linked motor neuron degeneration and cortical spine loss

Fig. 2

Sarm1 deletion reduces TDP-43Q331K-mediated NMJ degeneration. a. Representative images of immunofluorescent staining of NMJs in the gastrocnemius muscle from mice at different time points (Green = synaptophysin and β-III-tubulin co-stain, Red = α-bungarotoxin). Scale bar, 50 μm. (b-d) Percentages of fully innervated, partially innervated, and denervated NMJs in the gastrocnemius muscles of each genotype at different time points. b. At 5 weeks, ANOVA Interaction P < 0.0001. Pairwise comparisons: Fully innervated, Q331K-Sarm1+/− vs. Q331K-Sarm1−/−: ns P = 0.0520; Partially innervated, NTG vs. Q331K-Sarm1+/−: ** P = 0.0027; Q331K-Sarm1+/− vs. Q331K-Sarm1−/−: ns P = 0.5852; Denervated, NTG vs. Q331K-Sarm1+/−: ** P = 0.0024; Q331K-Sarm1+/− vs. Q331K-Sarm1−/−: ns P = 0.1107. c. At 3 months: ANOVA Interaction P < 0.0001. Pairwise comparisons: Fully innervated, Q331K-Sarm1+/− vs. Q331K-Sarm1−/−: ** P = 0.0032; Partially innervated, NTG vs. Q331K-Sarm1+/−: *** P = 0.0004; Q331K-Sarm1+/− vs. Q331K-Sarm1−/−: ns P = 0.5585; Denervated, Q331K-Sarm1+/− vs. Q331K-Sarm1−/−: * P = 0.0124. d. At 10 months: ANOVA Interaction P < 0.0001. Pairwise comparisons: Fully innervated, Q331K-Sarm1+/− vs. Q331K-Sarm1−/−: ns P = 0.1949; Partially innervated, Q331K-Sarm1+/− vs. Q331K-Sarm1−/−: ns P = 0.0711; Denervated, Q331K-Sarm1+/− vs. Q331K-Sarm1−/−: ** P = 0.0030. For (b-d) (n = 5 mice per genotype); ****P < 0.0001; two-way ANOVA followed by Holm-Sidak post-hoc test for pairwise comparisons. Error bars represent mean ± s.e.m

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