Skip to main content

Table 8 Summary of findings for the two outcomes from comparing perfusion fixation and immersion fixation identified in our review

From: Perfusion fixation in brain banking: a systematic review

Outcome Number of studies Number of brain samples Overall effect Quality of the evidence (GRADE)
Subjective histology quality 4 (1 randomized, 1 crossover) 116 Equal or superior histology quality in perfusion-fixed tissues when compared to immersion fixation of relatively large volumes of brain tissue
MODERATE
Long-term immunostaining quality 1 37 Slower long-term degradation of antigen staining quality for sensitive antigens in perfusion-fixed tissue
VERY LOW
  1. In the GRADE quality assessment system, there are four levels of quality: high, moderate, low, and very low