Table 8 Summary of findings for the two outcomes from comparing perfusion fixation and immersion fixation identified in our review
From: Perfusion fixation in brain banking: a systematic review
Outcome | Number of studies | Number of brain samples | Overall effect | Quality of the evidence (GRADE) |
|---|---|---|---|---|
Subjective histology quality | 4 (1 randomized, 1 crossover) | 116 | Equal or superior histology quality in perfusion-fixed tissues when compared to immersion fixation of relatively large volumes of brain tissue | ⨁⨁⨁⨀ MODERATE |
Long-term immunostaining quality | 1 | 37 | Slower long-term degradation of antigen staining quality for sensitive antigens in perfusion-fixed tissue | ⨁⨀⨀⨀ VERY LOW |