Fig. 1

LRP comprised of WT αsyn detected by selective antibody 3H11. a Western blot of 200 ng recombinant WT or A53T human αsyn protein probed with antibody 9C10 (residues 2–21) or antibody 3H11 (residues 43–63); A53T αsyn does not react with antibody 3H11. b Immunohistochemical staining with antibody 3H11 or 9C10 in αsyn transgenic mice. Using antibody 3H11, αsyn aggregates are extensively detected within line M20 mice overexpressing WT human αsyn but not in line M83 mice overexpressing A53T human αsyn demonstrating the histochemical specificity of this antibody. Labeling with antibody 9C10 depicts αsyn pathology in both types of αsyn transgenic mice. Scale bar 50 μm. c Immunohistochemical staining of tissue from the midbrain and hippocampus of a familial case of PD/LBD due to a heterozygous A53T mutation in SNCA. Antibody 9C10 detects both WT and A53T αsyn and detects abundant pathology in both regions; Antibody 3H11 only detects WT αsyn but also labels many pathologic inclusions, indicating that WT αsyn is recruited to aggregate by the presence of the A53T αsyn mutation. Arrowheads indicate LRP. All the sections depicted were treated with FA. Scale bar 50 μm