Fig. 2
From: Neuropathology of RAN translation proteins in fragile X-associated tremor/ataxia syndrome
Generation and characterization of new FMRpolyG antibodies. a Schematic showing epitopes for published (8FM, 9FM and 2 J7) and novel (NTF1, N-terminal FMRpolyG and CTF1, C-terminal FMRpolyG) antibodies against FMRpolyG. Epitope sequence is underlined (green line - 8FM and 9FM, blue line- 2 J7, red line - NTF1 and CTF1 FMRpolyG antibodies used in this paper). b Western blot using CTF1 and GFP antibodies in HEK293 cells transfected with a non-template control (NTC- lanes 1), EGFP-N1 plasmid (lanes 2), FMRpolyG100GFP (lanes 3) and ATG FMRpolyG99GFP (lanes 4). GAPDH used as loading control. c Immunocytochemistry of Mock, EGFP-N1 and ATG FMRpolyG100GFP transfected HEK293 cells using CTF1 antibody. Nuclei stained using DAPI. Scale in C = 10 μm. d Western blot using NTF1 and GFP antibodies in HEK293 cells transfected with a non-template control (NTC- lanes1), EGFP-N1 plasmid (lanes 2), FMRpolyG100GFP (lanes 3) and ATG FMRpolyG99GFP (lanes 4). GAPDH used as loading control. e Immunocytochemistry of Mock, EGFP-N1 and ATG FMRpolyG100GFP transfected HEK293 cells using NTF1 antibody. Nuclei stained using DAPI. Scale in E = 10 μm