Fig. 5

Gene expression levels are perturbed in spinal cord of aged TDP-∆C mice. a Venn diagram show deregulated genes in spinal cord (SC) of 700-day-old TDP-∆C mice identified by microarray analyses overlapped with ones directly regulated by TDP-43 (left). Representative deregulated genes, which are directly regulated by TDP-43 and related to motor dysfunction, in SC of 700-day-old TDP-∆C mice identified by microarray analyses (right). b Quantitative RT-PCR confirmed that levels of Notch1 and Adarb2 mRNA were downregulated in SC of 700-day-old TDP-∆C mice. The data are plotted as mean ± SD. c TDP-∆C reduced Notch1 mRNA levels in Neuro2a (N2a) cells. N2a cells were transfected with the indicated expression plasmids or siRNAs. After 24 h of the transfection, total RNA was isolated, and the Notch1 mRNA levels were quantified using quantitative RT-PCR. Note that both TDP-∆C overexpression (∆C) and Tardbp siRNA treatment (siTardbp) suppressed the Notch1 mRNA levels. ∆C F/L represents the TDP-∆C mutant lacking the nucleotide binding ability as indicated in Fig. 4c. The data are plotted as mean ± SEM (n = 3)