Fig. 1
Expression patterns of TERT, ETS-factors and activation of associated signaling cascades. a mRNA expression of ETS-factors and the ETS-downstream targets cyclin D1 and TERT were analyzed in the indicated genotypes. Means of three independent experiments are shown. Cyclin D1 mRNA expression of BRAF wild-type versus BRAFV600E mutated cell lines was quantified by unpaired student’s t-test (*p < 0.05). b Western blot analyses of cell lines with different BRAF and TERT promoter status as indicated are depicted. Proteins of S6 and MAPK pathway as well as selected ETS-factors and downstream targets are shown. Ratios between phosphorylated and total proteins as indicated were calculated after normalization to β-actin. wt = wild-type, mut = mutated