Fig. 6

Demyelination and axonal pathology was perceived in both the retina and optic nerve in EAE. a Demyelination (MBP) in the optic nerve was first observed 11 days post immunisation (dpi) progressing until 33 dpi where profound signs of myelin disorganisation and possible myelin debris were observed. b Axonal degeneration (NEFM) was observed from 11 dpi until 33 dpi in the optic nerve of EAE mice. c APP, important for axonal transport, appears to increase as early as 9 dpi and also persisted until the final observational time point in EAE optic nerve sections. In the retina, d NEFM appears to decrease in the IRL (arrows) and in the outer plexiform layer over time in EAE mice; beginning later (20 dpi) than in the optic nerve. e APP in the retina appears to increase (arrows) as early as 9 dpi similar to presentation in the optic nerve for EAE mice. EAE: experimental autoimmune encephalomyelitis, MBP: myelin basic protein, NEFM: neurofilament-M, APP: Alzheimer precursor protein, IRL: inner retinal layer, INL: inner nuclear layer, ONL: outer nuclear layer