Fig. 2

Inner retinal layer thickness increased at onset and decreased at later time points in EAE. a Boxplots of the inner retinal layer (IRL) thickness over time in EAE (yellow) and healthy controls (blue). b Optical coherence tomography retinal B-scan of healthy C57BL/6 J mouse with segmented layers including the IRL, inner nuclear layer and outer plexiform layer. Inset includes a diagram of the IRL composed of retinal ganglion cells (RGC). The retinal nerve fiber layer consists of the unmyelinated axons of the RGCs, with their cell bodies and dendrites located within the ganglion cell layer and inner plexiform layer respectively. * p-values for comparison between EAE and control mice (**** p < 0.00001, *** p < 0.0001), § p-value for comparison between baseline and recovery in EAE mice (§§§§ p < 0.00001). EAE: experimental autoimmune encephalomyelitis, dpi: days post immunisation. Scale bars: 200 μm