Fig. 4

CD4+ T-cell infiltration was prominent in early EAE and diminished in late EAE. a Schematics for optic nerve sample preparation. Each optic nerve was divided into 3 parts equally, as proximal, middle and distal to the optic nerve head. Each part had 3 regions of interest selected and total of 9 sections for each mouse were stained with interested antibodies. b CD4 staining in cross sectioned optic nerve of EAE mouse at PID 16 and PID 42, respectively. CFA group was from control of PID 42 since 2 CFA groups had no difference. White dashed lines delineate the ROI. c Quantification of CD4+ T-cells in EAE and CFA control mice. CD4+ T-cells for each animal was based on average of 9 sections from each mouse. Quantification is represented as mean ± SEM. Significance was compared by two-tailed, unpaired Student’s t-test with P < 0.05 considered as significant. *** P ≤ 0.001. ** P ≤ 0.01. Scale bar =50 μm