Fig. 3

Mechanism of ADCC bystander killing. a Perforin immunofluorescence of CHO-AQP4 and CHO-null cocultures after pre-coating with AQP4-IgG and incubation NK cells, as in Fig. 2b, with controls including control IgG and NK cells, and AQP4-IgG alone. White filled arrows indicate perforin on AQP4-expressing CHO cells, white open arrows show perforin on CHO-null cells. b Time-lapse imaging of coculture of CHO-AQP4 cells (labeled green with cell tracker) and CHO-null cells, pre-incubated for 30 min AQP4-IgG, then at indicated times after NK cell addition (for explanation see text and Additional file 2: Video S2)