Fig. 4

Arterioles were completely reperfused after recanalization and did not exhibit constrictions unlike downstream microvessels. a-c In vivo AOSLO imaging shows that the blood flow in large (b) as well as small arterioles (c) was restored to pre-ischemia levels (a) after re-opening of the central retinal artery. b illustrates one of the arterioles emerging directly from the central retina artery (41.8 μm), whereas the one c is a secondary arteriole (22.4 μm). Red fluorescence comes from the smooth muscle cells surrounding arterioles in NG2-DsRed mice, whereas green signal is generated by pseudo-coloring of erythrocyte motion in their lumina. Scale bars = 10 μm. For videos, please see the Additional files