Fig. 2

CitH3 levels and numbers of CitH3⁺ cells in peripheral blood after MCAO. Neutrophils were purified from peripheral blood after 12 h, 1 d, 2 d, and 4 d of MCAO. a-b Levels of MPO and CitH3 were examined in isolated neutrophils by immunoblotting (GAPDH was used as a loading control). c Triple immunofluorescent staining was conducted using anti-CitH3 antibody, anti-MPO antibody, plus DAPI. Scale bars in C-G represent 50 μm. The images in right panel are high magnification photographs of each image indicated as white box. d Ratio of CitH3⁺/DAPI⁺ cells are presented as means±SEMs (n = 4–8 from 4 to 8 animals). **p < 0.01 versus Sham-control