Fig. 1

Elevation of CitH3 levels in ischemic brains after MCAO. a Three brain regions used in the present study are indicated as follows, leptomeninges (Lm), cortex (Ct), and striatum (St). b-c Levels of CitH3 were examined in Lm, Ct, and St after 6, 12, 24, and 48 h of MCAO by immunoblotting (GAPDH was used as a loading control). Representative images are presented and results are presented as means±SEMs (n = 4). d Coronal brain sections were prepared after 24 h of MCAO and H&E stained. e-l Coronal brain sections were prepared after 12 h, 24, and 48 h of MCAO as indicated and immunofluorescent staining was conducted with anti-CitH3 antibody (e,f,j, and p) or with anti-CitH3, anti-Ly6g antibody, anti-RECA, anti-laminin, or DAPI (g-i and k-o). The photographs shown are representative of three independent experiments. The insets in D and H are high magnification photographs of each image. Scale bars in D-F and J represent 50 μm and those in G-I and K-O represent 20 μm. q CitH3⁺ cells in leptomeninges, cortex, and striatum (400 μm × 400 μm) were counted and results are presented as means±SEMs (n = 7–9 from 3 to 5 animals). **p < 0.01, ^##p < 0.01, ^$$p < 0.01 versus Sham-control