Fig. 4

IT delivery of rAAV1-HGF led to histological improvements of SMNs and NMJs in SOD1-G93A TG mice. a-g non-TG or TG mice at P60 were intrathecally injected with rAAV1-C or rAAV1-HGF. The LSCs (a-d) and TA muscles (e-f) were collected at P100, followed by IHC assay. a Representative image of SMNs. Antibodies specific to ChAT and NeuN were used to label SMNs. b Number of SMNs per ventral horn was counted and represented as a bar graph. c Proportion of SMNs per DAPI-positive cells was counted and represented as a bar graph. d Diameter of SMNs were measured and represented as a bar graph. In, Fig. 4b-d, one-way ANOVA was performed, followed by Tukey’s post-hoc test. e-f The shape and integrity of NMJs were determined as mentioned in Fig. 2 and represented as a bar graph. In Fig. 4e-f, two-way ANOVA was performed, followed by Tukey’s post-hoc test. In Fig. 4e, ^****p < 0.0001 for modified NMJ and ^####p < 0.0001 for pretzel-shaped NMJ. In Fig. 4f, ^****p < 0.0001 for fully innervated NMJ and ^####p < 0.0001 for partially innervated NMJ. g Relative mass of TA was calculated using total body mass and represented as a bar graph. For bar graphs, values are represented as mean ± SEM. For statistical analysis, one-way ANOVA was performed, followed by Tukey’s post-hoc test. For bar graphs, ^*p < 0.05, ^**p < 0.005, ^***p < 0.001, n.s. > 0.05. Scale bar: a = 50 μm