Fig. 2

Representative immunohistochemistry of induced αS pathology in M83^+/− mice neonatally injected at P0 with PBS, WT human αS fibrils, A53T human αS fibrils, or MSA brain lysates, and quantification of αS pathology. a Diagram of the αS protein with the seven imperfect amino acid repeats spanning the N-terminus and hydrophobic middle region in orange. Identified above are the αS antibodies utilized for assessment of αS pathology induction and their respective binding epitopes. b M83^+/− mice were injected at P0 with as described in “Material and Methods” and aged. Images showing αS inclusion pathology stained with anti-αS antibody 94-3A10 and 9C10 or pSer129 αS antibody 81A in the pons. Sections were also stained with an antibody to p62, a general marker in inclusion formation. c Quantification of αS pathology burden in M83^+/− mice induced with either MSA lysate, A53T αS fibrils, and WT αS fibrils, performed utilizing antibody 94-3A10 for pathology visualization. ** = p-value of <.01. **** = p-value of <.0001. Scale bar = 50 μm