Fig. 3
From: APOE genotype and sex affect microglial interactions with plaques in Alzheimer’s disease mice
Effects of APOE4 and sex on TREM2 expression levels near plaques. a Representative images showing ThioS-positive plaques (cyan) and Iba1-immunoreactive microglia (red) merged with TREM2 (green; with orange reflecting high co-localization with Iba1) in the HPC of EFAD mice. Scale bars = 10 μm. b Quantification of TREM2 load in the HPC in male (M) and female (F) EFAD mice with APOE3 (open bars) and APOE4 (filled bars) genotypes. c TREM2 load in plaque negative regions (> 100 μm away). d TREM2 intensity in Iba1-immunoreactive microglia proximal to sites of microglia-plaque interaction (normalized to distal regions). Numbers of mice analyzed per group are as follows: (b-c) male E3FAD (n = 6), female E3FAD (n = 7), male E4FAD (n = 5), and female E4FAD (n = 6). d male E3FAD (n = 4), female E3FAD (n = 6), male E4FAD (n = 4), and female E4FAD (n = 5). *** denotes p < 0.001, **** denotes p < 0.0001