Fig. 3

Reduced microglial plaque coverage in Aqp4 deficient 5xFAD mice. a Aβ, Iba1 and AQP4 immunofluorescence showing close association peri-plaque AQP4-labeled astrocyte processes and Iba1-labeled microglia in wild-type 5xFAD mouse. b Low (left panels) and high (right panels) magnification images of Iba1-labeled microglia surrounding plaques in Aqp4^+/+ and Aqp4^−/− 5xFAD mice. c Average number of Iba1-expressing cells in cortex of Aqp4^+/+ and Aqp4^−/− 5xFAD mice as determined by thresholding and object counting (difference not significant). d Fractional coverage of plaque cores with Iba1-labeled microglial processes displayed as a frequency distribution for all plaques from Aqp4^+/+ and Aqp4^−/− 5xFAD mice (left panel). Average extent of plaque containment by microglia as determined for at least 10 plaques in each individual mouse (right panel). (Aqp4^+/+ n = 8, Aqp4^−/− n = 5, **p < 0.01 by unpaired t-test)