Fig. 6

siRNA loss of LMTK2 reduces axonal levels and anterograde axonal transport of cdk5. a Neurons were either untreated (UT) or treated with control or LMTK2 siRNAs and then co-transfected with HA-cdk5 and DsRed to reveal neuronal architecture. Representative images of fixed neurons are displayed along with graph showing quantification of relative axonal HA-cdk5 levels detected using the epitope tag. Scale bar = 20 μm. Data were analysed by Welch’s ANOVA and Games-Howell post hoc test; N = 41–43, error bars are s.e.m., ***p < 0.001. b Representative kymographs showing axonal transport of EGFP-cdk5 in living neurons; for clarity anterogradely moving EGFP-cdk5 are illustrated alongside, time = 180 s. Graph shows quantification of anterogradely moving EGFP-cdk5 in the different treated neurons. Data were analysed by Welch’s ANOVA and Games-Howell post hoc test; N = 14–21, error bars are s.e.m., *p < 0.05