Fig. 5

siRNA loss of LMTK2 reduces axonal levels and anterograde axonal transport of p35. a Neurons were either untreated (UT) or treated with control or LMTK2 siRNAs and then co-transfected with EGFP-p35 and DsRed to reveal neuronal architecture. Representative images of fixed neurons are displayed along with graph showing quantification of relative axonal EGFP-p35 levels. Scale bar = 20 μm. Data were analysed by Welch’s ANOVA and Games-Howell post hoc test; N = 30–34, error bars are s.e.m., *p < 0.05, **p < 0.01. b Representative kymographs showing axonal transport of DsRed-p35 in living neurons; for clarity anterogradely moving DsRed-p35 are illustrated alongside, time = 180 s. Graph shows quantification of anterogradely moving DsRed-p35 in the different treated neurons. Data were analysed by Welch’s ANOVA and Games-Howell post hoc test; N = 17–26, error bars are s.e.m., *p < 0.05