Fig. 1
Radiotherapy is amenable to combine with Delta-24- in vitro and in vivo in the DIPG and pHGG models. a Evaluation by western blotting of the expression of viral proteins after Delta-24-RGD (10 MOIs) infection and subsequent irradiation (3 , 6 and 12 Gy) in TP54 and CHLA-03-AA. b Quantification of Delta-24-RGD replication in the indicated cell lines irradiated with different Gy doses. The viral titers were determined 3 days after infection at an MOI of 10 by an anti-hexon staining-based method in 293 cells and expressed as plaque-forming units (pfu) per milliliter. Data are shown as the mean ± SD of three independent experiments and analyzed with two-tailed Student-t test, not significant differences were found. c Cell viability analyses of irradiated cells at the indicated Gy doses alone (Mock; control with a mock infection) or in combination with Delta-24-RGD. Cell viability was assessed 5 days after irradiation and viral infection using an automatic cell counter that measures cell viability (life, death and total cells) with the standard trypan blue reaction. Data are shown as the percentage (the mean ± SD) of viability after treatments and relative to control cells (neither infected nor irradiated). Statistical significance were calculated using two-tailed Student-t test, ns, not significative; *, P < 0.05; **, P < 0.01; ***, P < 0.001. d Kaplan-Meier survival curves of nude mice bearing orthotopic DIPGs (TP54) or pHGG (CHLA-03-AA) tumors that were treated with either Delta-24-RGD (107 pfu), irradiation (4 Gys) or combined treatment. Log-rank test were used for statistical analyses