Fig. 4

Depletion of eIF4B and eIF4H1 selectively suppresses LDS-(G4C2)_EXP associated toxicity. a. Using GMR-GAL4, RNAi-mediated depletion of eIF4B or eIF4H1 in LDS-(G4C2)_EXP expressing flies results in reduced toxicity in both the external and internal eye: seen externally by recovered pigment and ommatidial structure, seen internally by recovered retinal tissue integrity. b. Blinded quantification of internal retina tissue was done by measuring the total surface area of tissue present and by measuring the depth of the tissue at the position where the optic chiasm occurs. n = 9–10 animals per genotype. c. eIF4B or eIF4H1 RNAi was expressed in (GR)36 flies (GMR-GAL4) and effects on GR-associated toxicity were observed in the external and internal eye. d. Blinded quantification of internal retina tissue. n = 5–9 animals per genotype. e. eIF4B or eIF4H1 RNAi was expressed in control flies (GMR-GAL4) and effects on the normal eye were observed externally and internally. f. Blinded quantification of internal retina tissue. n = 4 animals per genotype. For graphs, shown are individual data points representing 1 animal with mean ± SD. Statistics: one-way ANOVAs with Tukey’s multiple comparison correction, p-values **** < 0.0001, *** < 0.001, ** < 0.01, * < 0.05, no significance > 0.05. RNAi lines: control (JF01355), eIF4B (HMS04503), eIF4H1 (HMS04504). For full genotypes see Additional file 7: Table S1