Fig. 2

Ref1 reduction lowers TDP-43 RNA and protein abundance. a Ref1 Knockdown reduces TDP-43 mRNA levels in Drosophila heads. ***p < 0.001 two-tailed Student’s t-test. b Ref1 knockdown reduces TDP-43 protein levels in Drosophila heads. Western immunoblot analysis of human TDP-43 and β-Tubulin as loading control. *p < 0.05 two-tailed Student’s t-test. Genotypes for (a) and (b). Control RNAi: UAS-TDP-43/+; YH3-GAL4/UAS-control RNAi (JF01355). Ref1: UAS-TDP-43/+; YH3-GAL4/UAS-Ref1 RNAi (HMS01301). c Loss of Ref1 does not alter Tau mRNA levels. n.s. not statistically significant, two-tailed Student’s t-test. d Loss of Ref1 does not alter Tau protein levels. n.s not statistically significant, two-tailed Student’s t-test. Genotypes for (c) and (d). Control RNAi: YH3, UAS-Tau/ UAS-control RNAi (JF01355). Ref1 RNAi: YH3, UAS-Tau/ UAS-Ref1 RNAi (HMS01301). For all graphs, individual data points are shown with mean +/− standard deviation