Fig. 8
Hallmarks of synucleinopathies are recapitulated in cultured astrocytes infected with MSA. a Immunohistochemical detection of α-synuclein inclusions (red) in patient sample MSA2 (basal ganglia) (left), brain tissue from TgM83 mice inoculated with MSA2 (middle), and primary astrocyte culture infected with TgM83-passaged MSA2 prions. b Representative immunographs of high-power confocal microscopy of brain tissues and astrocyte culture from (a) immunostained for phosphorylated α-synuclein S129 (red) and ubiquitin (green; second row), p62 (green; third row), or amyloid detecting dye–FSB (green; fourth row). Nuclei were stained with DAPI (blue). Scale bars, (a) 200 μm, (b) 10 μm