Fig. 5

α-Synuclein inclusions in MSA-infected astrocytes are ubiquitinated and co-localized with p62 or contain α-synuclein phosphorylated at Y125. Representative immunographs of TgM83^+/+ primary astrocytes exposed to 0.5% secondary passaged MSA₂ brain homogenate for 48 h and immunostained at 0, 7, 14, and 21 days post-exposure (dpe) for a ubiquitin (green) and pSyn (S129) (red), b p62 (green) and pSyn (S129) (red), and c αSyn (green) and pSyn Y125 (magenta). Merge channels are shown, and c insets of dashed areas are shown in the bottom row. Nuclei were stained with DAPI (blue). Scale bars, 20 μm