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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Replication of multiple system atrophy prions in primary astrocyte cultures from transgenic mice expressing human α-synuclein

Fig. 3

Subpassage of MSA prions in TgM83 cultured astrocytes. a Schematic representation of cell homogenate preparation and media collection from MSA-infected TgM83+/+ astrocyte cultures at 21 dpe. Naïve astrocytes were then exposed to the cell homogenate or medium for 48 h, cultured up to 21 days, and analyzed for pSyn (S129) at four time points. b Quantification of TgM83+/+ (black diamonds) and TgM83+/− (grey diamonds) astrocytes exposed to MSA-infected cell homogenate and TgM83+/+ astrocytes cultured with media previously cultured with MSA-infected astrocytes (white diamonds). Astrocytes were immunolabeled for pSyn (S129), and the signal intensity was normalized by cell count. Data are plotted with mean and analyzed by one-way ANOVA followed by Tukey’s multicolumn comparison test: ****, P < 0.0001; **, P = 0.0017

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Acta Neuropathologica Communications

ISSN: 2051-5960

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