Fig. 5

PAE results in elevated SGC activation and cytokine immunoreactivity in the dorsal root ganglia (DRG). Immunoreactivity of (a) SGCs (as measured by GFAP immunoreactivity), (b) IL-1β, and (c) IL-10 are determined for the ipsilateral L4-L6 DRG of PAE and Sac minor CCI vehicle injected rats at Day 4 post-injection (Day 32 after minor CCI) (rats from data shown in Fig. 1). (a) SGCs activation is significantly upregulated following minor CCI in both Sac and PAE rats. Additionally, compared to Sac rats, PAE rats have heightened SGCs activation. b Significant increases are observed following minor CCI in vehicle injected PAE rats compared to Sac rats with sham or minor CCI. c Sac-sham rats with vehicle display significantly greater IL-10 immunoreactivity compared to PAE-sham rats with vehicle rats. N = 5 rats per group. Asterisks indicate p < 0.05. d - f Representative images of SGCs, IL-1β, and IL-10 immunoreactivity used in IHC analysis are shown for Sac/ Sham/Veh, Sac/1-Sut/Veh, PAE/Sham/Veh, PAE/1-Sut/Veh, at 20x. “1-sut CCI” = “minor CCI”. N = 5 = 7 rats per group. Asterisks indicate p < 0.05