Fig. 3

MiR-204 expression levels in the medulloblastoma cell lines before and after exogenous expression and effect of restoration of miR-204 expression on proliferation and anchorage-independent growth. a. MiR-204 expression in the parental cells (control) and the established polyclonal populations P1, P2 of the indicated medulloblastoma cell line with (+) and without (−) doxycycline induction. b. Effect of miR-204 expression on growth of medulloblastoma cells studied by the MTT assay. Y-axis denotes growth of the vector control and polyclonal populations P1, P2 of the indicated medulloblastoma cell line after doxycycline treatment as a percentage of the growth of the corresponding un-induced population of the cells. c & d. Effect of miR-204 expression on the anchorage-independent growth of medulloblastoma cell lines studied by the soft agar colony formation assay. Y axis denotes the number of soft agar colonies formed by the P1, P2 polyclonal populations and the Vector control population of the indicated cell line upon doxycycline induction of miR-204 expression (c) and in (d), as a percentage of those formed by the un-induced cells. **, *** indicate p ≤ 0.001 and p ≤ .0001 respectively