Fig. 6

[F-18]-AV-1451 and [F-18]-MK-6240 phosphor screen and high resolution autoradiography photomicrographs of brain slices containing occipital (#13) (a), temporal (#9) (b) and entorhinal (#14) (c) cortices from AD cases (left panels). Middle and right panels (a and b) show immunostaining of adjacent sections with PHF-1 antibody (kind gift of Dr. Peter Davies) and anti-Aβ antibody (1:500, mouse, clone 6F/3D, Dako), respectively. [F-18]-AV-1451 (left panels a and c) and [F-18]-MK-6240 (left panel b and right panel c) high resolution nuclear emulsion autoradiography showed a strong cortical accumulation of silver grains in temporal cortical layers III and V (a and b), and in layers II and IV of the entorhinal cortex (c) in AD brains mirroring the laminar pattern of tangles on adjacent slices as revealed by PHF-1 immunostaining (middle panels) rather than the more scattered plaque distribution pattern revealed by Aβ immunostaining (right panels). Abbreviations: AD = Alzheimer’s disease; IHC = immunohistochemistry. Scale bars = 200 μm (a and b) 50 μm (c)