Fig. 3

TRIM32 mutant myoblasts exhibit a decrease in cell proliferation. a Quantification of number of primary myoblast (proliferation rate) at 1, 4 and 8 days growing in proliferation medium from family A patients (n = 2), family B patients (n = 2), healthy controls (n = 6) and disease controls (2 LGMD2B, X-EDMD) (n = 3). The proliferation rate is reduced in TRIM32^V591M and TRIM32^{N217S/F568del} myoblasts compared with controls. Data from 12 to 45 independent fields were analyzed per time point. Mean ± SEM; Kruskal-Wallis with Dunn’s multiple comparisons test. b. Analysis of primary myoblast proliferation at 8 days growing in proliferation medium using Ki67 as a marker of dividing cells from family A patients (n = 2), family B patients (n = 2) and healthy controls (n = 6). Immunofluorescence showing double staining, desmin (red) and Ki67 (green). Nuclei were counterstained with Topro 3 (blue). Quantification of Ki67⁺ cells revealed a progressive decrease in the percentage of proliferating TRIM32^V591M and TRIM32^{N217S/F568del} myoblasts compared with controls. Data from 14 to 41 independent fields were analyzed per condition. Mean ± SEM; Kruskal-Wallis with Dunn’s multiple comparisons test. Scale bar, 50 μm