Fig. 6
From: Tau as a mediator of neurotoxicity associated to cerebral amyloid angiopathy
Tau oligomers cell specificity in Tg-FDD mice. a-e Fluorescent microscopic images of Tg-FDD brain section triple immunostaining for amyloid (Thio-S, green), Tau oligomers (T22, red), and astrocytes (GFAP, cyan). Tau oligomers immunoreactivity showed close association with astrocytes and astrocytic processes (Merge). Colocalization analysis (CC) was performed to determine pixel intensity correlation between T22 and GFAP signals. White pixels indicate colocalization between T22 and GFAP signal (e, white pixels). f-j Triple immunostaining for amyloid (Thio-S, green), Tau oligomers (T22, red), and microglia (Iba1, cyan). Tau oligomers are not associated to microglia surrounding vascular amyloid (Merge). Colocalization analysis confirmed the lack of correlation between T22 and Iba1 signal (j). k-o Triple immunostaining for amyloid (Thio-S, green), Tau oligomers (T22, red), and neurons (NeuN, cyan). Tau oligomers were found associated to neurons in areas where vascular amyloid was not detected. White pixels indicate colocalization between T22 and NeuN signal (o, white pixels). All figures were obtained from 18 months old Tg-FDD mice cerebral cortex. Scale bar 25 μm