Fig. 2

ADan oligomers promoted tau phosphorylation and cellular toxicity that is tau dependent. a WB using anti-ADan antibody revealed the formation of multimers from ADan recombinant peptide after 2, 8, 24, and 48 h of stirring. WB using the conformational anti-oligomers antibody F11G3 confirmed the formation of ADan recombinant oligomers after 48 h of stirring. HMW = High Molecular Weight, LMW = Low Molecular Weight. b WB analysis of lysates from HEK tau-P301L cells exposed to PBS, ADan monomer or oligomers. c Graph showing WB quantification of p-tau S396/S404/ total tau ratio. For p-tau S396/S404 analysis; *p < 0.05, One-way ANOVA with Turkey’s correction. For quantification, error bars represented ± SD (n = 3). Values of phospho-tau S396/S404/total tau ratio in cells treated with PBS were considered as 100%. d Cytotoxicity exerted by ADan oligomers in cells, measured by MTT reduction, depends on human tau-P301L expression induced by Dox. For PBS-oligomer analysis in (Dox+); *p < 0.0001, Unpaired Student’s t test. For monomer-oligomer analysis in (Dox+); *p < 0.0001, Unpaired Student’s t test. For quantifications, error bars represented ± SEM (n = 6). MTT values were originally obtained as absorbance at 570 nm. For graphic purposes, MTT reduction in cells treated with PBS were considered as 100%