Fig. 1

Lama5 expression is abrogated in mural cells in α5-PKO mice. a Representative images of laminin-α2 (green) and laminin-α5 (red) staining in the cortex of control and α5-PKO mice. Scale bar = 100 μm. b Representative western blotting and quantification of laminin-α2 and laminin-α5 levels in the cortex of control and α5-PKO mice. n = 4. c Representative images of smooth muscle actin-α (SMA, green) and laminin-α5 (red) staining in primary mural cells isolated from control and α5-PKO brains. Scale bar = 50 μm. d Quantification showing the lack of laminin-α5 expression in primary mural cells isolated from α5-PKO brains. n = 6 independent experiments with at least 50 cells examined in each experiment. Student’s t-test, ***p < 0.001, compared to controls